Apparent affinity of LapDdual or LapDEAL for c-di-GMP.

aFor LapDdual variants containing the A602E mutation, maximum binding at the highest c-di-GMP concentrations was significantly lower compared to LapDdual lacking this point mutation. Assuming similar binding capacity of the various proteins, the data of the A602E-containing constructs of LapDdual could not be fitted accurately, and estimated Kd values are much larger than the highest c-di-GMP concentration used in the titrations.bLapDEAL shows an overall weaker dimerization propensity than LapDdual, which affects the stability of the nucleotide-bound state.n.d., not determined.