Lillis Dust Sequencing Data

16S sequence data and metadata sampled from dust in rooms in the Lillis Hall building at the Unviersity of Oregon.
Three sets of files are included in this data archive.
dust_mapping.txt - a tab-delimited mapping file for use with QIIME software. Includes information on primer and barcode sequences associated with each sample.*** note that in the raw sequence data, a combinatorial or dual-index barcoding approach was used - the first 6bp of the barcode are on the forward read, and the final 6bp of the barcode are on the reverse read. The barcodes will need to be combined into a single 12bp construct prior to parsing into samples.
dust_metadata.txt - a tab-delimited file containing metadata associated with each sample.
dust_metadata_description.txt - a tab-delimited file with description of each column in the metadata file.
dust.fastq.tgz.part-xx - raw sequence data in FASTQ format, produced by two sequencing runs using Illumina MiSeq 150bp paired-end. Due to file size limitations, the original tar/gzip archive has been split into seven parts. To recombine and uncompress the sequence files, the following command works in a Linux/Unix environment:cat dust.fastq.tgz.part-a*| tar xz

16S序列数据及其元数据，由俄勒冈大学Lillis Hall建筑内房间中的灰尘样本采集而来。该数据档案包含三组文件。
dust_mapping.txt - 用于QIIME软件的制表符分隔的映射文件。包含与每个样本相关的引物和条形码序列信息。**请注意，在原始序列数据中，采用了组合式或双索引条形码方法——条形码的前6个碱基位于正向读取中，而条形码的最后6个碱基位于反向读取中。在解析为样本之前，需要将条形码合并为一个12个碱基的结构。
dust_metadata.txt - 包含与每个样本相关元数据的制表符分隔文件。
dust_metadata_description.txt - 包含元数据文件中每列描述的制表符分隔文件。
dust.fastq.tgz.part-xx - 以FASTQ格式产生的原始序列数据，由两次使用Illumina MiSeq 150bp配对端测序运行生成。由于文件大小限制，原始tar/gzip归档已被分割成七个部分。在Linux/Unix环境中重新组合和解压缩序列文件，以下命令有效：cat dust.fastq.tgz.part-a*| tar xz