Conservation of phosphorylated residues, cyclin recognition motifs, and Cdk substrate motifs among SIRT1 orthologs of 12 different species.

Comparison of SIRT1 sequences by “BLAST 2 Sequence” among species, in which the protein sequence of the SIRT1 ortholog was available. Listed in order of the similarity to human SIRT1, from top to bottom. For species for which ELM motif data were available (BOLD), motif conservation was analyzed as well. For cyclin recognition motif, “ok” stands for a complete conservation of the motif, “?” for a partial conservation, and “ng” for loss of conservation. SP (14–15, 26–28, 47–48, 545–546) and TP (530–531) fit the minimal Cdk substrate motif, and the sequence between amino acids 537–543 fits the full Cdk substrate motif.Abbreviations: cyc, cyclin recognition motif; Cdk, full Cdk-substrate motif.