Transcriptomics and epigenomics of E15.5 female germ cells (PRJCA033553)

Epigenetic reprogramming is widespread and highly active during gametogenesis, which is usually involving in the expression of critical genes. The expression of genes couple with transcription activation, and the transcriptional regulation by transcription factors predetermines protein translation for biological processes. In this study, we found that EHMT1-mediated re-establishment played crucial roles in the progression of meiosis in female germ cells. EHMT1 deficient female mice were nearly infertile due to the arrest of zygotene in embryonic germ cells, which was caused by down-regulated expression of key meiotic genes. Furthermore, we identified transcription suppressor for meiotic genes by combining RNA-seq, Cut&Tag seq analysis of H3K9me2 and luciferase reporter assays.