Small RNA next generation sequencing of miRNAs in MCF7 cells derived mammosphere treated with withaferin A. Small RNA next generation sequencing of miRNAs in MCF7 cells derived mammosphere treated with withaferin A

In this study, we reporte the altered miRNAs expression in the human breast cancer cell line (MCF7) derived mammosphere in response to withaferin A IC50 concentrations (2 µM).. Overall design: Withaferin A is a major pharmacological active constituent of Withania somnifera (Solanaceae). In this study we were aimed to identify the diffentially expressed known/novel miRNAs using next generation sequencing (NGS) in withaferin A treated MCF7 derived mammosphere. For this, MCF7 cells were initially treated with withaferin A with 2 µM concentration (experimental groups) and without treated MCF7 cells (the control group) were allowed to form spheres and incubated at 37 ºC under a 95% humid environment in an incubator having 5% CO2 for five days. Three samples were assigned for each group.