NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018

<p>This dataset includes NCBI Accessions for Raw sequence data for 16S rRNA amplicon microbiomes.</p> <p>The NCBI BioProject number is PRJNA555077 which covers 1547 samples of 16S libraries where the metadata are also available but the sequences themselves are still embargoed. [2019-08-15]</p> <p>Samples were collected at 15 m by SCUBA diving in the Caribbean near Curacao, Belize, Cayman Islands and St. Croix, in 2009 and 2017-2018. Samples were cleaned, frozen whole, or placed in a DNA/RNA preservation buffer and shipped back to the University of New Hampshire where DNA was extracted and metagenetic, shotgun or cDNA sequencing was conducted.</p> <p>Data was quality controlled and analyzed in spreadsheets for descriptive statistics, and statistical software was used for bioinformatic analyses.</p>本数据集包含16S rRNA扩增子微生物组原始测序数据的NCBI登录号（NCBI Accessions）。 本数据集对应的NCBI生物项目（BioProject）编号为PRJNA555077，涵盖1547份16S文库样本，其配套元数据已可公开获取，但原始测序序列仍处于保密限制状态。[2019-08-15] 样本采集工作于2009年、2017-2018年实施，通过水肺潜水在加勒比海库拉索、伯利兹、开曼群岛及圣克罗伊岛附近的15米水深海域完成。采集后的样本经清洁处理后，或进行整体冷冻保存，或置于DNA/RNA保存缓冲液中，随后被运送至新罕布什尔大学，在该校完成DNA提取并开展宏基因组、鸟枪法或cDNA测序实验。 数据集已完成质量控制，通过电子表格进行描述性统计分析，并借助专业统计软件开展生物信息学分析。