Impact of TCF-1 de-repression on effector gene expression programs [RNAseq_Tcf7del22+17]

The transcription factor TCF-1 is downregulated during effector differentiation of CD8+ T cells. However, how its downregulation impacts gene expression in different effector- and memory-biased populations remains unclear. Here, we generate mice in which TCF-1 fails to become downregulated in differentiated effector cells and probe the transcriptional impact of TCF-1 retention in different T cell differentiation states bybulk RNA-sequencing of KLRG1+ effector T cells. RNA-sequencing of WT + Cd19 RNP control P14 TCR trangenic cells and Tcf7∆22kb + Tcf7+17kb RNP P14 TCR transgenic cells isolated on 8 days post infection with LCMV-Armstrong.