Designing and implementing programmable depletion in sequencing libraries with DASHit
收藏NIAID Data Ecosystem2026-03-11 收录
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资源简介：
The DASH technique takes advantage of the programmability of CRISPR/Cas9 to deplete unwanted high-abundance sequences from cDNA libraries. Because desired depletion targets vary by sample type, we designed DASHit, a computer program that outputs an optimal DASH guide RNA set given a sequencing dataset. Here we describe the program, along with an updated DASH protocol, and show results of depletion with DASHit-designed gRNA sets for three different species.
创建时间：
2019-12-24
