Supplementary data for article: "Human precision-cut intestinal slices as a new model for testing induction potency of drugs influencing the gene, protein or activity of P-glycoprotein (ABCB1)"

Supplementary data for article: "Human precision-cut intestinal slices as a new model for testing induction potency of drugs influencing the gene, protein or activity of P-glycoprotein (ABCB1)." In figure S1 is presented effect of increasing concentrations of rifampicin (RIF) on P-glycoprotein (ABCB1) mRNA levels in human precision-cut intestinal slices (hPCIS). Figure S2 contains information about quenching effect of RIF on the fluorescence of rhodamine123 in acetonitrile:water solution (2:1). Figure S3 and S4 present information about ABCB1 and Cytochrome P450 3A4 (CYP3A4) mRNA levels, respectively, over the course of 48 hours incubation in control and RIF-treated hPCIS. Last figure S5 demonstrates pregnane X receptor (PXR) (A) and retinoid X receptor alpha (RXRA) (B) mRNA levels over the course of 48 hours incubation in control hPCIS.本补充数据对应论文《人精准切割肠切片作为检测调控P-糖蛋白（P-glycoprotein，ABCB1）的基因、蛋白质或活性的药物诱导效能的新型模型》。图S1展示了不同浓度利福平（rifampicin，RIF）对人精准切割肠切片（human precision-cut intestinal slices，hPCIS）中ABCB1的mRNA表达水平的影响。图S2包含利福平（RIF）对体积比为2:1的乙腈-水溶液中罗丹明123荧光的淬灭效应相关信息。图S3与图S4分别展示了对照组与利福平处理的人精准切割肠切片中，ABCB1与细胞色素P450 3A4（Cytochrome P450 3A4，CYP3A4）的mRNA水平在48小时孵育过程中的变化情况。最后一幅图S5展示了对照组人精准切割肠切片中，孕烷X受体（pregnane X receptor，PXR）（面板A）与维甲酸X受体α（retinoid X receptor alpha，RXRA）（面板B）的mRNA水平在48小时孵育过程中的变化情况。