ZEBRA-mediated translocation of PABPC and regulation of the intranuclear distribution of translocated PABPC by ZEBRA are mechanistically distinct.

As described for Fig. 9, 293 cells transfected with empty vector or expression vectors for wild-type (WT) and mutant ZEBRA in the presence or absence of transfected BGLF5 were fixed and stained with antibodies specific for ZEBRA and PABPC. Cells expressing WT or mutant ZEBRA proteins were scored for ZEBRA-induced alterations to the intranuclear distribution of PABPC, and for ZEBRA-induced translocation of PABPC from the cytoplasm to the nucleus.