Phosphoproteomic study of HMMR loss in neuroblastoma cells

This is processed dataset from a mass spectrometric analysis of phosphopeptides extracted from KELLY neuroblastoma cells. It compares peptides from cells with wild type HMMR genes and cells where the HMMR is inactivated using CRISPR/Cas9. The analysis aimed to understand how intracellular signaling was altered after the removal of HMMR to understand the potential functional role of HMMR in these tumour cells. the signalling networks in which HMMR was involved in these tumour cells. The are four cell types used: KELLY (parental), KC17 (subject to CRISPR/Cas9 but with intact HMMR genes), KA5 and KA14 (subjected to CRISPR/Cas9 and with out of frame mutations in HMMR, and not expressing the protein). We examined peptides in KA5 and KA14 that show significantly different phosphorylation from those found in the KELLY and KC17. The MS-482 datafile contain several outputs including heatmaps, KSEA analyses plus detailed individual peptide data after processing as described in Thompson, E. M., et al. (2022). FEBS Open Bio. DOI: 10.1002/2211-5463.13418.本数据集为经处理的质谱分析数据集，样本取自KELLY神经母细胞瘤细胞中提取的磷酸化肽段。本研究对比了携带野生型HMMR基因的细胞与经CRISPR/Cas9介导HMMR基因灭活的细胞的肽段表达情况。本次分析旨在阐明HMMR缺失后细胞内信号通路的改变，以解析HMMR在该类肿瘤细胞中的潜在功能及其参与的信号调控网络。本数据集共使用四种细胞系：KELLY（亲本细胞）、KC17（经CRISPR/Cas9编辑但HMMR基因完整的细胞）、KA5与KA14（经CRISPR/Cas9编辑且HMMR基因存在移码突变、不表达HMMR蛋白的细胞）。本研究检测了KA5和KA14细胞中，相较于KELLY及KC17细胞存在显著磷酸化差异的肽段。MS-482数据文件包含多种分析输出结果，包括热图、激酶集富集分析（KSEA）以及按照Thompson, E. M., et al. 2022年发表于《FEBS Open Bio》的文献中所述流程处理得到的单个肽段详细数据，文献DOI：10.1002/2211-5463.13418。