Data from: In meso crystallization: compatibility of different lipid bicontinuous cubic mesophases with the cubic crystallization screen in aqueous solution

Attached file provides supplementary data for linked article.

In meso crystallization uses bicontinuous cubic lipidic mesophases as matrices for the crystallization of membrane proteins. In this work, we look at the impact of a screen specifically marketed as compatible with the cubic mesophase, the Cubic crystallization screen (Emerald BioSystems), on the cubic mesophases formed by three different lipids: monoolein, monopalmitolein, and phytantriol. The Cubic screen was found to be compatible with cubic mesophase retention under most crystallization conditions for all three lipids studied. The effect of the individual components comprising the multicomponent screen was deconvoluted in two ways. Initially, the effect of specific poly(ethylene glycol) (PEG) and salt components on the cubic mesophase was determined using small-angle X-ray scattering (SAXS). The effect of high-molecular-weight (M w) PEG was shown to dominate the phase behavior within the screen. The effect of additional salts present within the screen becomes important for low Mw PEG molecules. Finally, a recently developed multiple linear-regression modeling method was shown to deconvolute the effect of individual components within the screen effectively.

本附件文件为关联文章提供补充数据集。
中相结晶法采用双连续立方脂质介观相作为膜蛋白结晶的载体基质。本研究针对一款专为适配立方相介观相而开发的结晶筛选试剂盒——立方相结晶筛选试剂盒（Cubic crystallization screen，Emerald BioSystems出品），考察其对三种不同脂质（单油酰甘油、单棕榈油酰甘油及植烷三醇）所形成的立方相介观相的影响。
实验结果表明，在所考察的三种脂质的多数结晶条件下，该立方相筛选试剂盒均可维持立方相介观相的稳定存在。
本研究通过两种方式对该多组分筛选试剂盒的各单一组分的影响进行了解析分离。首先，借助小角X射线散射（SAXS）技术，明确了特定聚乙二醇（PEG）与盐类组分对立方相介观相的影响。
实验证实，高重均分子量（M_w）的PEG组分对筛选试剂盒内的相行为起主导作用。
而对于低重均分子量的PEG组分而言，筛选试剂盒中额外添加的盐类组分的影响则更为关键。
最后，本研究证实，新近开发的多元线性回归建模方法可有效解析分离该筛选试剂盒内各单一组分的影响。