Data underlying the publication: Model-based aberration corrected microscopy inside a glass tube

Data for the paper "Model-based aberration corrected microscopy inside a glass tube".<br>Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In non-standard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably impairing high-resolution imaging. Correcting such large aberrations using standard adaptive optics can be challenging: existing solutions are either not suited for strong aberrations, or require extensive feedback measurements, consequently taking a significant portion of the photon budget. We demonstrate that it is possible to pre-compute the corrections needed for high-resolution imaging inside a glass tube based on a priori information only. Our ray-tracing based method achieved over an order of magnitude increase in image contrast without the need for a feedback signal.<br>Contents:1. 3-D 2PEF scans of fluorescent beads inside a glass tube.2. Brightfield microscopy images of the glass tube.3. Parameter scans of the phase correction patterns.4. Sensorless AO scans (Zernike mode scans).5. Ray-traced model-based phase correction patterns for glass tube.6. Code to recreate figures from the paper from raw data.7. Protocol to create tube samples.

本数据集为论文《基于模型的玻璃管内像差校正显微成像》的配套数据。
显微镜物镜仅在其设计工况下，方可实现近衍射极限的成像性能。在非标准几何条件下，例如厚盖玻片或曲面结构时，会产生严重像差，不可避免地劣化高分辨率成像效果。采用标准自适应光学技术校正这类大幅像差颇具挑战：现有解决方案要么无法适配强像差，要么需要大量反馈测量，进而耗费大量光子预算。我们证实，仅需基于先验信息，即可预先计算出玻璃管内高分辨率成像所需的校正量。我们提出的基于光线追踪的方法无需反馈信号，即可将图像对比度提升一个数量级以上。
数据集内容：
1. 玻璃管内荧光微球的三维双光子激发荧光（2PEF）扫描数据
2. 玻璃管明场显微图像
3. 相位校正模式的参数扫描数据
4. 无传感器自适应光学（AO）扫描（泽尼克模式（Zernike mode）扫描）
5. 适用于玻璃管的光线追踪模型相位校正模式
6. 用于从原始数据复现论文中各图表的代码
7. 玻璃管样本制备方案