Transcriptional responses of GII.4 human norovirus infection in jejunal human intestinal enteroids

The goal of this project was to evaluate intestinal epithelial responses to GII.4 human norovirus infection. Jejunal (J2 and J11) human intestinal enteroid cultures infected with GII.4 human norovirus. Mock- and gamma-irradiated GII.4 (gGII.4) inoculated cultures were used as controls. We performed RNA sequencing (RNA-Seq) to determine transcriptional responses to GII.4 at 6, 10 and 24 hours post-inoculation (hpi) in comparison to mock-inoculated cultures (3 hpi) or inoculation with gGII.4 HuNoV (6, 10 and 24 hpi).