Iain M. Porter, Sarah E. McClelland, Guennadi A. Khoudoli, Christopher J. Hunter, Jens S. Andersen, Andrew D. McAinsh, J. Julian Blow, Jason R. Swedlow (2011) CIL:13377, Homo sapiens. CIL. Dataset

z-focal series through Hela cell in metaphase illustrating MCAK localization in Bod-1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.

通过Hela细胞有丝分裂中期展示的z-聚焦系列，描绘了Bod-1siRNA细胞中MCAK（微管结合蛋白A激酶）的定位。细胞经过对照组（本图像）或Bod1（CIL: 13378）siRNA转染。经过72小时后，细胞在加入monastrol处理3小时，随后释放至含有MG132的培养基中孵化1小时后进行固定。细胞被染色以标记总的分裂中期着丝粒相关激酶（MCAK）群体。MCAK在磷酸化状态下定位于着丝粒内部，但在去磷酸化状态下则集中于纺锤体微管上。