Genome-wide CRISPR screen of post-transcriptional regulators of IL6 3'UTR. Homo sapiens

The aim of this research is to uncover novel post-transcriptional mechanisms regulating IL6 3'UTR. IL6 is one of the most essential cytokines and its expression is tightly controlled during acute and chronic inflammation. IL6 mRNA is known to be regulated by a variety of RNA binding proteins to adequately control immune response. However, our understanding of posttranscriptional regulation of this gene is still incomplete. To comprehensively address this issue, we performed a genome-wide screen to identify posttranscriptional regulators of IL6 3 prime UTR using CRISPR gRNA library. This study will provide us with a comprehensive list of potential regulators of IL6 3 prime UTR.