Oral cancer cell sequencing after coculturing with neurons

To explore the tumor cell-intrinsic signaling altered after SCC15 cells coculturing with trigeminal ganglion (TG) neurons, the total RNA of SCC-15 cells was collected from the coculture groups 1 and 2 as well as the control group and performed RNA-seq. The coculture group 1 was set with TG sensory neurons in the lower chamber and tumor cells in the upper inserts, while the coculture group 2 was set with the coculture medium from coculture group 1 in the lower chamber and the tumor cells in the upper inserts. The control group was set with SCC15 cells only.