Additional file 1: of Platform-independent gene expression signature differentiates sessile serrated adenomas/polyps and hyperplastic polyps of the colon

Table S1. The list of qRT-PCR primers for signature genes. Table S2. Normalized expression levels (median and MAD) obtained by qRT-PCR from 45 independent FFPE samples and the classification result obtained using the 13 genes molecular signature with different sample normalizations. Table S3. Raw expression levels of 13 genes in the molecular signature obtained by qRT-PCR from 45 independent FFPE samples. Table S4. The list of 139 genes that were DE between SSA/Ps and both HP and CR samples. Table S5. The list of 172 genes, exclusively DE between SSA/P and HP samples. Table S6. The list of 1014 genes, exclusively DE between SSA/P and CR samples. Table S7. The list of 105 genes, DE in three comparisons. Table S8. Number of common genes between three different platforms. Table S9. Class probabilities assigned using empirical approach, normal approximation, shrunken centroid classifier (independent of the summary metric), and the Cantelli’s inequality lower bound when the 18-gene signature from Table 2 is used. Table S10. Class probabilities assigned using empirical approach, normal approximation, shrunken centroid classifier (independent of the summary metric), and the Cantelli’s inequality lower bound when the 16-gene signature from Table 2 is used. Table S11. Class probabilities assigned using empirical approach, normal approximation, shrunken centroid classifier (independent of the summary metric), and the Cantelli’s inequality lower bound when the 13-gene signature from Table 2 is used. (XLSX 275 kb)表S1 特征基因的实时逆转录定量聚合酶链反应（quantitative reverse transcription PCR，qRT-PCR）引物列表 表S2 基于45份独立福尔马林固定石蜡包埋（Formalin-Fixed Paraffin-Embedded, FFPE）样本的qRT-PCR检测结果，经归一化处理后的基因表达水平（中位数及中位数绝对偏差（Median Absolute Deviation, MAD）），以及采用13基因分子特征结合不同样本归一化方法得到的分类结果 表S3 从45份独立FFPE样本的qRT-PCR检测结果中提取的分子特征所包含的13个基因的原始表达水平 表S4 139个差异表达（Differentially Expressed, DE）基因列表，这些基因在无蒂锯齿状腺瘤/息肉（Sessile Serrated Adenoma/Polyps, SSA/P）与增生性息肉（Hyperplastic Polyp, HP）、无蒂锯齿状腺瘤/息肉与结直肠（Colorectal, CR）样本中均存在差异表达 表S5 仅在无蒂锯齿状腺瘤/息肉与增生性息肉样本间存在差异表达的172个基因列表 表S6 仅在无蒂锯齿状腺瘤/息肉与结直肠样本间存在差异表达的1014个基因列表 表S7 在三组比较中均存在差异表达的105个基因列表 表S8 三种不同检测平台间共有的基因数量 表S9 采用表2中的18基因分子特征时，通过经验法（empirical approach）、正态近似法（normal approximation）、收缩质心分类器（shrunken centroid classifier，与汇总指标无关）及康特利不等式下界（Cantelli’s inequality lower bound）得到的分类概率 表S10 采用表2中的16基因分子特征时，通过经验法、正态近似法、收缩质心分类器（与汇总指标无关）及康特利不等式下界得到的分类概率 表S11 采用表2中的13基因分子特征时，通过经验法、正态近似法、收缩质心分类器（与汇总指标无关）及康特利不等式下界得到的分类概率 （XLSX格式，文件大小275 KB）