Budding index of agar cultures of S.cerevisiae, 210 min after inoculation – in control and after 120 min induction with various inductors (see fig. 4D for the whole budding curve on this medium).
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The experiment scheme is given in fig. 3A. Inductor – S.cerevisiae cultures of various age, in YPD (suspension) and on YPD-agar. Recipient – S.cerevisiae culture 15 min after inoculation.Medium content: CH3COONa 0,1%+KH2PO4 0,1%+MgSO4 0,05%+CaCl2 0,01%+NaCl 0,01%+agar 3%, pH5,5.
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2015-12-02
