Genome-wide analysis of LPS-responsive gene expression in human macrophages

Analysis of gene expression changes in human macrophages in response to LPS in the absence or presence of a CD11b agonist LA1. The hypothesis being tested in the present study was that LA1 mediated CD11b activation suppresses TLR signaling. Total RNA obtained from human macrophages subjected to LPS for 8 hours in vitro in the absence or presence of LA1. Control cells were treated with either vehicle (DMSO) or with LA1 alone. Cells were treated with vehicle DMSO (1% DMSO), LA1 (20 μM), LPS (50 ng/mL), or LPS (50ng/mL) and LA1 (20 μM) for 4h.