Acute phase protein and vitamin D concentration in dogs with multicentric lymphoma

Abstract This study aimed to evaluate the serum concentration of vitamin D (25-Hydroxyvitamin D) and acute phase proteins (APPs; alpha-1 acid glycoprotein, haptoglobin, transferrin, ceruloplasmin, albumin, IgA, IgG and alpha-1 - antitrypsin) as potential biomarkers for prognostic and therapy response in dogs with multicentric lymphoma submitted to the CHOP (Cyclophosphamide, Doxorubicin, Vincristine and Prednisone) chemotherapy protocol. Thirteen dogs with multicentric lymphoma classified as high grade by cytology were included in the treatment group (GL), while ten healthy dogs were included in the control group (GC). Serum was collected in the weeks T0, T5 and T10 of CHOP chemotherapy protocol, for the GL group, and in a single collection, for the GC group. All the collected samples were evaluated for the APPs and vitamin D concentrations through electrophoresis and chemiluminescence methods, respectively. Diagnostic and staging tests were performed for all the dogs in the GL group, and included cytopathology, histopathology and immunohistochemistry of the affected lymph node. Of these dogs, 9 achieved a complete response and 4 a partial response to the treatment. Data analysis was performed with the R software. The results demonstrated that serum concentrations of IgA, haptoglobin and α1-acid glycoprotein were significantly different between the groups and also between the different chemotherapy times analyzed (p<0.05), indicating that these proteins can be considered as sensitive biomarkers for lymphoma in dogs. Furthermore, the α1-acid glycoprotein showed prognostic value for the disease, with 63% specificity. However, vitamin D concentration was not correlated with prognosis of the dogs with lymphoma.摘要：本研究旨在评估维生素D（25-Hydroxyvitamin D）与急性期蛋白（APPs，包括α1-酸性糖蛋白、结合珠蛋白、转铁蛋白、铜蓝蛋白、白蛋白、免疫球蛋白A、免疫球蛋白G及α1-抗胰蛋白酶）作为接受CHOP（环磷酰胺、多柔比星、长春新碱、泼尼松）化疗方案治疗的多中心淋巴瘤犬的预后及治疗反应的潜在生物标志物的价值。本研究纳入13只经细胞学判定为高级别的多中心淋巴瘤犬作为治疗组（GL），另纳入10只健康犬作为对照组（GC）。治疗组犬分别在CHOP化疗方案的T0、T5、T10周采集血清，对照组犬仅单次采集血清。所有采集的样本分别通过电泳法与化学发光法检测急性期蛋白与维生素D浓度。对治疗组所有犬只均开展诊断与分期检测，包括受累淋巴结的细胞病理学、组织病理学及免疫组织化学检查。其中9只犬对治疗达到完全缓解，4只达到部分缓解。数据分析采用R软件完成。结果显示，治疗组与对照组间，以及不同化疗时间节点间，免疫球蛋白A、结合珠蛋白及α1-酸性糖蛋白的血清浓度均存在显著差异（P<0.05），表明这些蛋白可作为犬淋巴瘤的敏感生物标志物。此外，α1-酸性糖蛋白对该病具有预后价值，其特异性达63%。然而，维生素D浓度与淋巴瘤犬的预后并无相关性。