Raw nuclear magnetic resonance data of human linker histone H1x lacking the C-terminal domain (NGH1x) and trajectory data of nanosecond molecular dynamics simulations of GH1x- and NGH1x-chromatosomes (NMR data).

Human linker histone H1 is an important role player in the packaging of DNA. H1 has a tripartite structure: an evolutionarily conserved central globular domain that adopts a winged-helix fold, flanked by the highly variable and intrinsically unstructured N- and C-terminal domains. This dataset consists of raw 2D and 3D BEST-TROSY NMR data recorded on Bruker Avance III HD spectrometers, operating at 700 or 950 MHz 1H frequency, and equipped with cryogenically cooled triple-resonance (HCN) probes and pulsed z-field gradients at 5'C (278 K). Data were recorded for NGH1x [residues 1 – 120 of human H1x; UniProtKB: Q92522(H1X_HUMAN) consisting of the N-terminal and globular domains] in 20 mM sodium phosphate (‘low salt’) or 20 mM sodium phosphate + 1 M sodium perchlorate (‘high salt’). Data can be analyzed using NMR spectra analysis software such as Sparky, CCPNMR, etc. A description of the data folders is given in the file "NMR Description of data folders.pdf".人类连接组蛋白H1在DNA包装过程中发挥关键作用。H1具有三组分结构：进化保守的中央球状结构域呈翼螺旋折叠构象，两侧为高度可变且内在无序的N端与C端结构域。本数据集包含在布鲁克（Bruker）Avance III HD核磁共振波谱仪上采集的原始二维及三维BEST-TROSY核磁共振（NMR）数据，该类波谱仪的1H共振频率为700 MHz或950 MHz，配备低温冷却三重共振（HCN）探头，并搭载5°C（278 K）下运行的脉冲z场梯度线圈。数据采集对象为NGH1x——即人类H1x的第1至120位残基（对应UniProtKB登录号Q92522[H1X_HUMAN]，包含N端结构域与球状结构域），分别在20 mM磷酸钠缓冲液（"低盐"条件）或20 mM磷酸钠+1 M高氯酸钠缓冲液（"高盐"条件）中完成采集。此类数据可通过Sparky、CCPNMR等核磁共振波谱分析软件进行解析。数据文件夹的详细说明请参见文件"NMR Description of data folders.pdf"。