Use of a GnRH synthetic analog (buserelin) for estrous induction in female dogs

ABSTRACT The aim of this study was to evaluate the effectiveness of a GnRH synthetic analog, as an estrous inducer in female dogs when administered during the anestrous phase, and to evaluate the pregnancy rate achieved through natural copulation. For this purpose, ten female dogs of different breeds were used. The subjects received buserelin by intramuscular injections at a dose of 2,1mcg when female dogs weighed up to 10kg (Group 1) and of 4,2mcg when the dogs weighed above 10kg (Group 2). Of the ten subjects, only three presented estrus after a single injection of buserelin: two dogs from Group 1 and one dog from Group 2 on average 7±1.29 days. The remaining seven dogs were given a second dose of buserelin, equal to the first administration. Of these, three belonged to Group 1 and four to Group 2. Four of these dogs exhibited clinical signs of estrus within, on average 9±7.3 days from the second injection. The seven female dogs that did enter estrus were fertilized successfully through natural copulation. The administration of buserelin was effective in inducing estrus in female dogs during the anestrous phase, with a maximum of two administrations.摘要 本研究旨在评估合成促性腺激素释放激素（Gonadotropin-Releasing Hormone，简称GnRH）类似物作为发情诱导剂用于乏情期母犬的效果，并评估通过自然交配获得的受孕率。为此，本研究选用10只不同品种的母犬作为实验对象：当母犬体重不超过10kg时（第1组），以2.1μg的剂量肌内注射布舍瑞林（buserelin）；当母犬体重超过10kg时（第2组），给药剂量调整为4.2μg。10只受试犬中，仅3只在单次注射布舍瑞林后出现发情症状，其中第1组2只、第2组1只，平均发情诱导时长为7±1.29天。剩余7只犬接受了第二次布舍瑞林注射，给药剂量与首次一致，其中第1组3只、第2组4只；该7只犬中有4只在第二次注射后平均9±7.3天内出现发情临床症状。所有成功进入发情期的7只母犬均通过自然交配实现成功受孕。本研究结果证实，在乏情期母犬中使用布舍瑞林进行发情诱导，最多两次给药即可取得良好效果。