Varying subpopulation sizes of ICE<i>clc</i> transfer competent cells in <i>P. knackmussii</i> B13–1343 P_int<i>-egfp</i> grown on different carbon sources.

110 mM of carbon source in minimal medium (see Methods). 2Average ICEclc transfer competent subpopulation of cells (percent of total) determined from biological triplicates, expressing egfp from Pint ± standard deviation. Sampled 15 - 20 h after onset of stationary phase. Determined via R command find.sub.pop(…) in Default mode. 3* and ** indicate significant differences at P<0.05 and P<0.01, respectively, as determined by the Welch Two Sample t-test.