Expression data from whole blood from healthy human individuals

Blood is one of the most common biospecimens used in clinical and translational research. However, minimizing the variability in collection and processing of human blood samples remains a challenge. Delaying plasma or serum isolation after phlebotomy (processing delay) can cause perturbations of numerous analytes used in research studies and clinical practice. We used microarrays to investigate the effects of time delay and temperature changes on whole blood transcriptomics. Blood from healthy donors was subjected to processing delays that are commonly encountered in blood sample collection: 2hr RT, 5hr RT, 5hr 4C, and 15hr (ON) 4C.