Totten Glacier ocean & sediment DNA (IN2017_V01)

Genetic data underlying the paper 'From the Surface Ocean to the Seafloor: Linking Modern and Paleo-genetics at the Sabrina Coast, East Antarctica (IN2017_V01)' by Armbrecht et al. In this study, we provide the first taxonomic overview of the modern and ancient marine bacterial and eukaryotic communities of the Totten Glacier region, East Antarctica, using a combination of 16S and 18S rRNA amplicon sequencing (modern DNA) and shotgun metagenomic (sedimentary ancient DNA, sedaDNA) analyses, respectively. We explore environmental and geochemical variables that drive these biodiversity patterns. Our data show considerable differences between eukaryote and bacterial signals detected via DNA analyses in the water column vs. the sediments. Organisms that are well represented in deeper waters appear are to have a higher likelihood of becoming preserved in the sediments. The study provides the first assessment of DNA transfer from ocean waters to sediments, while also providing a broad overview of the biological communities occurring in the climatically important Totten Glacier region.

本数据集为Armbrecht等人发表的论文《从表层海洋到海底：南极东部萨布里纳海岸现代与古遗传学关联研究（IN2017_V01）》提供了遗传数据支撑。本研究针对南极东部托滕冰川区域，首次完成现代与古代海洋细菌及真核生物群落的分类学概述，分别采用16S rRNA扩增子测序（16S rRNA amplicon sequencing）与18S rRNA扩增子测序（18S rRNA amplicon sequencing，均针对现代DNA），以及鸟枪宏基因组学（shotgun metagenomic，针对沉积物古DNA（sedimentary ancient DNA, sedaDNA））进行分析。本研究还探究了驱动该区域生物多样性分布模式的环境与地球化学变量。研究数据表明，水体柱与沉积物中通过DNA分析检测到的真核生物与细菌信号存在显著差异。在深水层中丰度较高的生物，其在沉积物中得以保存的概率也更高。本研究首次评估了DNA从海水向沉积物的转移过程，同时全面概述了栖息于气候关键区域托滕冰川的生物群落组成。