Mus musculus Genome sequencing and assembly

By performing PacBio long-read sequencing of normal thymus tissues from three groups of mice (7 days old, 4 weeks old, and 4 months old), we identified a total of 136,104 transcripts that were mapped on average to 16,667 annotated genes and 22,465 novel loci. Furthermore, we identified the presence of different expression genes (DEG) and isoforms in the three groups of samples, which are enriched in inflammation-related signaling pathways.