CD100 stimulation on primary hepatocyte

To determine the effects of CD100 stimulation on hepatocyte, we have employed mRNA microarray expression profiling as a discovery platform to identify genes with the potential to response to CD100 stimulation, for evaluating the CD100 effects on the metabolism of hepatocyte. The primary hepatocyte, but not the hepatocellular cell lines, was used for the CD100 stimulation experiment ex vivo, and different expressing genes was identified that distinguished among MOCK, CD100 stimulated and TNF-a-stimulated (as a positive control) groups. The hepatocytes (10E6 each well) were stimulated by 1μg BSA (MOCK), 1μg CD100 or 200ng TNF-a (as positive control). The gene expression of MOCK group, CD100-stimulated group and TNF-a-stimulated group were measured by microarray at 24 hours after exposure to different stimulators. Three independent experiments were performed at each time using the primary hepatocytes purchased from Zenobio (Research Triangle, Raleigh-Durham, NC, US).