DNA targets for TrwC-mediated integration.
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Donor cells are II1 with pCIG1077 and pR6K::oriTP oriTw, and recipient cells are DH5α containing the indicated recipient plasmid, with (+TrwA) or without (−TrwA) plasmid pET3a::trwA. Data are the mean of at least three independent assays. Frequency is given in integrants per donor.
创建时间:
2012-01-23




