SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in October 2014

Flow cytometry data was collected in October 2014, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术（Flow cytometry）数据采集于2014年10月，采集区域为澳大利亚南部近海海域。本研究的核心目标是为澳大利亚南部大陆架海域的生态系统建立基础背景资料，阐明上升流与高盐径流事件对微生物群落的影响，最终构建该区域的生物地球化学模型。
采样工作作为南澳大利亚综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目的组成部分，在RV Ngerin号考察船上开展。每次巡航期间，均对叶绿素荧光最大值（Deep Chlorophyll Maximum, DCM）层的物理、化学与生物学特性进行调查。本次研究已针对超微型浮游植物、细菌与病毒完成流式细胞术数据采集。
本研究共布设6个主要采样站点：其中5个站点位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）以及SAM5CB/B5（35.008°S，135.198°E）；剩余1个为离岸站点B1（36.188°S，136.178°E），地处袋鼠岛西南海域。
需说明的是，结合站点间距（14~25海里）、陆架中层流的平均流速（0.01 m·s⁻¹）以及考察船的平均航速（约9节），可推断各站点所采集的水体分属不同水团。此外，研究团队还偶尔在袋鼠岛国家参考站（National Reference Station, NRS，35.832°S，136.447°E）以及发生高盐径流的南澳大利亚斯宾塞湾口锚系站点（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）采集了补充样本。