Genome assembly of Fujian oyster

Three sequencing and assembly technologies were integrated to perform the de novo assembly. Firstly, the genome of C. angulata was sequenced with 64-fold coverage of 150 bp Illumina paired-end sequencing reads to estimate the genome size and complexity. The C. angulata genome has a high level of heterozygosity (2.6%), and the genome size was estimated at 548-megabase (Mb) (supplementary fig. 1B). Then, 76.3-fold coverage of HiFi reads from the Pacific Biosciences Sequel II system were assembled into 372 contigs with a contig N50 of 12 Mb. Lastly, 97.7-fold coverage of the Hi-C library reads was employed to help generate a chromosome-level assembly, and the final genome assembly of C. angulata comprised 624 Mb with scaffold N50 of 60 Mb.