RUB262_genotypes

Microsatellite genotypes at locus RUB262. This is a tab-delimited text file in the format produced by Applied Biosystems GeneMapper software version 3.7. Each sample*locus genotype is represented in one row. The "Sample Name" column gives names of samples that directly correspond to those used in "species_chloroplast_latlong.csv". The "Well" column indicates the location of the sample on the DNA dilution plates. The "Marker" column indicates the microsatellite marker. The "Panel" column indicates groups of markers that were run simultaneously on the ABI3100 capillary sequencer. The "Dye" column indicates the fluorescent tag used to detect the PCR fragments: Y for NED, G for HEX, and B for FAM. The "Allele" columns contain the names of all alleles detected for each genotype. Cells are left blank if fewer alleles were detected than the number of columns. A value of -9 for Allele 1 indicates missing data. The "Height" columns indicate the height of the peak representing each allele, and the "Size" columns indicate the exact size of the DNA fragment as calculated by GeneMapper. A value of "TRUE" in the "AE" column indicates that the genotype was manually edited in GeneMapper, although all genotypes were visually inspected. The R package "polysat" can read these file directly, and will use the "Sample Name", "Marker", and "Allele" columns, and will ignore all other columns.RUB262位点的微卫星基因型数据。本数据集为制表符分隔的文本文件，格式适配应用生物系统（Applied Biosystems）GeneMapper软件3.7版本的输出格式。每个样本-位点基因型占一行。“样本名称（Sample Name）”列对应的样本名称，与“species_chloroplast_latlong.csv”文件中使用的样本完全一致。“孔位（Well）”列标注了样本在DNA稀释板上的位置。“标记（Marker）”列标注了所用的微卫星标记。“分组（Panel）”列标注了在ABI3100毛细管测序仪上同时上机运行的标记组。“荧光染料（Dye）”列标注了用于检测PCR扩增片段的荧光标签：Y代表NED，G代表HEX，B代表FAM。“等位基因（Allele）”列存储了每个基因型检测到的所有等位基因名称。若检测到的等位基因数量少于列数，则对应单元格留空。若“等位基因1（Allele 1）”列取值为-9，则代表该数据缺失。“峰高（Height）”列代表对应等位基因的峰高值，“片段大小（Size）”列则为GeneMapper软件计算得到的DNA片段精确长度。“AE”列取值为“TRUE”时，代表该基因型已在GeneMapper软件中完成手动校正，且所有基因型均经过人工目视核查。R语言包“polysat”可直接读取该文件，且仅会调用“样本名称”“标记”及“等位基因”三列数据，其余列将被自动忽略。