DRIVE raw data

These are the raw and genelevel data from the DRIVE pooled shRNA screen. It contains read counts for 398 cancer cell lines screened for 7’837 genes with an average of 20 shRNAs per gene. The data are provided as a RDS file that can be read in R using the readRDS function. They are formatted as a data.frame containing a unique identifier of the individual shRNA, its sequence, the plasmid counts, the sample counts, an experiment identifier, the name of the pool and the cell line name. Plasmid counts refers to shRNA abundance in the library input, and sample counts refers to NGS counts of individual shRNAs post drop-out screen. To query and download processed data, an interactive tool is available: https://oncologynibr.shinyapps.io/drive/本数据集包含来自DRIVE混合shRNA（short hairpin RNA）筛选的基因水平原始数据。该数据集涵盖针对7837个基因（每个基因平均对应20条shRNA）的398株癌细胞系的读段计数数据。本数据集以RDS文件格式提供，可通过R语言的readRDS函数读取。该数据集采用数据框（data.frame）格式存储，包含单条shRNA的唯一标识符、序列信息、质粒计数、样本计数、实验标识符、筛选池名称以及细胞系名称。其中质粒计数代表初始文库中shRNA的丰度，样本计数则为敲除筛选完成后单条shRNA的下一代测序（Next-Generation Sequencing, NGS）读段计数。如需查询并下载处理后的数据，可使用交互式工具：https://oncologynibr.shinyapps.io/drive/