Characterization of the human testicular primary cells

In this study, we carried out experiments using commercially available human testicular primary cells - hTPc (Celprogen, CA, USA), obtained from a 25-years old Caucasian man with normal karyotype 46,XY. To characterize the hTP cells, we used the commercial total RNA obtained from normal testicular tissues with preserved spermatogenesis (n=3) (Clontech Laboratories; Agilent Life Technologies, Carlsbad, CA, USA; Ambion Life Technologies, Carlsbad, CA, USA) Overall design: We performed a comparative analysis of gene expression profiles by RNA-seq between: human testicular primary cells (hTPc) (n=3), the human testicular primary cells from another vial (hTPcIM) (n=3) and human testis with normal spermatogenesis (n=3)