Data from: Microsatellite loci for dreissenid mussels (Mollusca: Bivalvia: Dreissenidae) and relatives: markers for assessing exotic and native populations

We developed and tested 14 new polymorphic microsatellite loci for dreissenid mussels, including the two species that have invaded many freshwater habitats in Eurasia and North America, where they cause serious industrial fouling damage and ecological alterations. These new loci will aid our understanding of their genetic patterns in invasive populations as well as throughout their native Ponto-Caspian distributions. Eight new loci for the zebra mussel Dreissena polymorpha and six for the quagga mussel D. rostriformis bugensis were compared with new results from six previously published loci to generate a robust molecular toolkit for dreissenid mussels and their relatives. Taxa tested include D. polymorpha , D. r. bugensis , D. r. grimmi , D. stankovici , the “living fossil” Congeria kusceri , and the dark false mussel Mytilopsis leucophaeata (the latter also is invasive). Overall, most of the 24 zebra mussel (N=583) and 13 quagga mussel (N=269) population samples conformed to Hardy-Weinberg equilibrium expectations for the new loci following Bonferroni correction. The 11 loci (eight new, three previously published) evaluated for D. polymorpha averaged 35.1 alleles and 0.72 mean observed heterozygosity per locus, and 25.3 and 0.75 for the nine loci (six new, three previously published) developed for D. r. bugensis . All but three of these loci successfully amplified the other species of Dreissena , and all but one also amplified Congeria and Mytilopsis . All species and populations tested were significantly divergent using the microsatellite data, with neighbor-joining trees reflecting their evolutionary relationships; our results reveal broad utility for resolving their biogeographic, evolutionary, population, and ecological patterns.本研究为斑蚌类（dreissenid mussels）开发并验证了14个全新的多态性微卫星位点（microsatellite loci），所覆盖的两个目标物种已入侵欧亚大陆与北美诸多淡水生境，并造成严重的工业污损损害与生态系统改变。这些新位点将助力我们解析入侵种群以及其原生蓬蒂-里海（Ponto-Caspian）分布区内种群的遗传模式。 本研究针对斑马贻贝（Dreissena polymorpha）开发了8个新位点，针对斑驴贻贝（D. rostriformis bugensis）开发了6个新位点，并结合6个已发表位点的新检测结果，构建了一套适用于斑蚌类及其近缘类群的稳定可靠的分子工具包。 本次检测的类群包括斑马贻贝（D. polymorpha）、斑驴贻贝（D. r. bugensis）、D. r. grimmi、D. stankovici、"活化石"库氏藏蚌（Congeria kusceri）以及暗伪贻贝（Mytilopsis leucophaeata），其中暗伪贻贝同样为入侵物种。 整体而言，经邦费罗尼校正（Bonferroni correction）后，24组斑马贻贝种群样本（N=583）与13组斑驴贻贝种群样本（N=269）中，多数新位点的检测结果符合哈迪-温伯格平衡（Hardy-Weinberg equilibrium）预期。 针对斑马贻贝检测的11个位点（含8个新位点与3个已发表位点），每个位点平均包含35.1个等位基因（alleles），平均观测杂合度（observed heterozygosity）为0.72；针对斑驴贻贝开发的9个位点（含6个新位点与3个已发表位点）则分别平均包含25.3个等位基因与0.75的平均观测杂合度。 除3个位点外，其余位点均可成功扩增斑蚌属（Dreissena）其余物种的靶序列；除1个位点外，其余位点均可成功扩增藏蚌属（Congeria）与伪贻贝属（Mytilopsis）的靶序列。 基于微卫星数据，所有检测类群与种群均呈现出显著的遗传分化，邻接树（neighbor-joining trees）清晰反映了它们的演化亲缘关系；本研究结果表明，这套工具包可广泛用于解析斑蚌类的生物地理、演化、种群及生态模式。