supplementary data High expression of DNAJA1 (HDJ2) predicts unfavorable survival outcomes in breast cancer.pdf

Supplemental Table
S1. Sample information sheet of the commercial tissue microarray (TMA)
used in the study TMA (BC081116b): Breast carcinoma tissue microarray with
adjacent breast tissue, containing 100 cases of breast invasive ductal
carcinoma and 10 cases of adjacent breast tissue, single core per case
(Biomax Inc. Rockville, MD, USA)

Supplemental Table
S2. Sample information sheet of the commercial tissue microarray (TMA)
used in the study TMA (BR486): Breast invasive ductal carcinoma tissue
microarray with matched adjacent normal mbreast tissue, containing 16 cases
of breast invasive ductal carcinoma with matched adjacent normal breast
tissue, triplicate cores per case (Biomax Inc. Rockville, MD, USA)

Supplemental Table
S3. Primers used for mutation analysis

Supplemental Table
S4. GEO and Array Express datasets used for survival (OS, RFS and DMFS)
analyses by KM-plotter

Supplemental Table
S5. Associations between DNAJA1 protein expression and clinical
characteristics of invasive breast carcinoma samples

Supplemental
Figure S1. Representative photographs from DNAJA1 immunohistochemistry
study performed with commercial tissue microarrays.

Supplemental
Figure S2

Kaplan-Meier plots of
OS (a), RFS (b) and DMFS (c) in breast cancer subtypes according to DNAJA1
mRNA expression.

Supplemental
Figure S3

(a) Heterozygous SNP
(rs2297218) in the promoter region of DNAJA1 in SK-BR-3 cell line. “SK-BR-3
(F)”: Sequencing with forward primer; “SK-BR-3 (R)”: Sequencing with reverse
primer; “Ref. Seq”: DNAJA1 genomic sequence (NCBI Gene ID: 3301) (b) The
position of the SNP relative to the promotor

motifs and location
of the primers are depicted. Promotor motifs are estimated by EPD “Search
Motif Tool” from -2000 bp to 100 bp relative to TSS (P value = 0.001)
(https://epd.epfl.ch//index.php).

Supplemental
Figure S4

Bisulfite-modified
sequences of the DNAJA1 gene analysed by COBRA assay.

Supplemental
Figure S5

Kaplan-Meier plot of Overall
Survival in breast cancer according to DNAJA1 protein expression (n=65)
(Uniprot ID: P31689) (Györffy et al., 2010; Tang et al., 2018)