Mechanistic study on direct and indirect thyroid toxicity in male Wistar rats - Serum Metabolomics

The present oral toxicity study in male Wistar rats is focusing on direct and indirect thyroid<br> toxicity. Biomaterials from this study were used to generate transcriptomics, proteomics,<br> and metabolomics data sets. Featuring a time- and concentration-resolved design<br> including recovery after treatment, this study allows investigating adaptive versus adverse<br> effects in several dimensions.<br> Two well-described test chemicals, i.e., Phenytoin and Propylthiouracil, were administered<br> at each two dose levels to male Wistar rats for 2 (Subset A) and 4 weeks via the diet<br> (Subset B). In addition, a recovery period of 2 weeks without test substance application was<br> included, in order to monitor reversibility of the induced effects (Subset C).<br> During the course of the study, blood samples were taken for metabolome analyses.