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Gene expression profiling by CRISPR/Cas13d-mediated CTSL knockdown in 293FT cells

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https://www.ncbi.nlm.nih.gov/sra/SRP375280
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To examine the potential off-target effects by CRISPR/Cas13d-mediated CTSL knockdown in 293FT cells Overall design: 293FT cells were transfected with non-targeting pre-gRNA control(gCtrl) or pre-gRNA targeting CTSL(gCTSL) expression plasmid in combination with Cas13d expression plasmid for 24h. RNA-seq was performed to characterize the differential expressed genes between gCtrl and gCTSL groups.
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2022-09-09
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