Deciphering the circadian regulation of the nodulation in the model legume, Medicago truncatula

In order to determine how gene expression in nodules changes diurnally and to compare them with gene expression in roots, we used RNAseq to quantify gene expression in nodules and roots of plants transferred from a diurnal 12/12h light-dark photoperiodic cycle to constant light. We sampled nodules and roots immediately after the transfer and then after every 3 hrs for the first day, then every 6 hrs for the second day (13 time-points- 0h, 3h, 6h, 9h, 12h, 15h, 18h, 21h, 24h, 30h, 36h, 42h, 48h)