Primer sequences used for amplification.
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Underlined restriction enzyme sites - CATATG: NdeI, AAGCTT: HindIII, GGATCC: BamHI, GAATTC: EcoRI, ACGCGT: MluI, ACTAGT: SpeI, TTAATTAA: PacI, CTCGAG: XhoI. C1 and C2 are alternate primers for cloning of irtB in pET28a and pGEX4T1. Refer Fig. 2A and B for schematic representation of the amplicons.
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2015-12-02
