Comparative transcriptome analysis revealed the biocontrol mechanism of Paenibacillus polymyxa NSY50 against tomato Fusarium wilt

In the process of greenhouse tomato cultivation, due to high multiple cropping index, high temperature and humidity, and relatively closed environment, it is easy to reduce the soil biodiversity and increase the pathogen quantity, and eventually lead to serious leaf, fruit and soil-borne diseases. Out of them, tomato Fusarium wilt is one of the most spread soil-borne fungus caused by Fusarium oxysporum f. sp. lycopersici (Fol). At present, chemical fungicides is still the principal method to control Fol. A large number of chemical pesticides will cause soil and water pollution and seriously endanger human health. Biological control as a safe, eco-friendly, efficient and low cost method has become the focus of Fusarium wilt control. In 2016, Dr. Du, a member of our research team, isolated a biocontrol agent P.polymyxa NSY50 from vinegar residue compost and used proteomics to reveal that NSY50 actively regulate the composition of rhizosphere microbial community to induce cucumber defense response against Fusarium oxysporum f. sp. cucumerinum. In this study, the comparative transcriptome analysis was used to analyze the changes of differentially expressed genes (DEGs) in tomato root tissues induced by NSY50 after Fol infection, and preliminarily analyzed the pathway of NSY50 mediated tomato resistance response to Fusarium wilt.