SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in November 2008

Flow cytometry data was collected in November 2008, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data has been collected for picophytoplankton (not bacteria and viruses this cruise).Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), B4 (35.168S, 135.418E) and B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术（Flow Cytometry）数据采集于2008年11月，采集区域为澳大利亚南部近海海域。本研究的核心目标为：建立澳大利亚南部大陆架海域生态系统的基础背景资料，阐明上升流与高盐溢流事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作依托澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目，在RV Ngerin号科考船上完成。每次科考巡航期间，研究人员均对叶绿素荧光最大值（chlorophyll fluorescence maximum, FM）层的物理、化学及生物学特性开展调查。本次采集的流式细胞术数据针对微微型浮游植物（picophytoplankton，本次巡航未采集细菌与病毒相关样本）。本研究共完成6个主要采样站位的样本采集：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、B4（35.168°S，135.418°E）及B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛西南海域。需说明的是，结合站位间距（14~25海里）、陆架中层海流平均流速分量（0.01 m·s⁻¹）及科考船平均航速（约9节）可推断，各站位采集的水体团并不相同。此外，研究人员偶尔会在袋鼠岛国家参考站（National Reference Station, NRS，35.832°S，136.447°E）以及发生高盐溢流的SA斯宾塞湾口锚站（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）采集补充样本。