Dataset for: Myricetin as a Radioprotective Biomodifier for Irradiated Human Root Dentin: Implications for Restorative Care in Head and Neck Oncology

This dataset was generated to test the hypothesis that ionizing radiation, as used in head and neck cancer radiotherapy, compromises the mechanical, enzymatic, and adhesive properties of human root dentin, and that myricetin (MYR), a naturally occurring flavonoid, can mitigate these deleterious effects through antioxidant, anti–matrix metalloproteinase (MMP), and collagen-stabilizing mechanisms.

The dataset includes quantitative measurements obtained from an in vitro experimental model using human third molars. Root dentin specimens were allocated to non-irradiated controls or irradiated groups exposed to single cumulative doses of 20, 40, or 60 Gy. For each radiation condition, samples were evaluated either without treatment or after application of 600 µM myricetin for 120 seconds. Mechanical behavior was assessed by three-point bending tests, providing flexural strength, strain, and elastic modulus values. Enzymatic degradation was evaluated through a fluorometric assay measuring total MMP activity in dentin. Adhesive performance was analyzed using microtensile bond strength testing of resin–dentin interfaces, performed both at 24 hours and after thermocycling, along with failure mode classification.

The data show a clear dose-dependent effect of radiation, characterized by significant reductions in flexural strength, elastic modulus, and microtensile bond strength, accompanied by increased dentin deformation and marked activation of MMPs, particularly at 60 Gy. Myricetin-treated specimens demonstrated partial or complete recovery of biomechanical parameters, significant suppression of MMP activity, and preservation of adhesive performance comparable to non-irradiated controls, even after aging. Failure mode distributions shifted toward cohesive failures within the resin in myricetin-treated groups, indicating improved interfacial integrity.

All raw data are provided in spreadsheet format, organized by experimental group and outcome variable, allowing independent statistical reanalysis or secondary use. This dataset can be used to explore radiation-induced alterations in dentin biomechanics, enzymatic activity, and adhesion, as well as to support the development and validation of biomodification strategies aimed at improving restorative outcomes in irradiated dental tissues.