Dataset for: Myricetin as a Radioprotective Biomodifier for Irradiated Human Root Dentin: Implications for Restorative Care in Head and Neck Oncology

This dataset was generated to test the hypothesis that ionizing radiation, as used in head and neck cancer radiotherapy, compromises the mechanical, enzymatic, and adhesive properties of human root dentin, and that myricetin (MYR), a naturally occurring flavonoid, can mitigate these deleterious effects through antioxidant, anti–matrix metalloproteinase (MMP), and collagen-stabilizing mechanisms. The dataset includes quantitative measurements obtained from an in vitro experimental model using human third molars. Root dentin specimens were allocated to non-irradiated controls or irradiated groups exposed to single cumulative doses of 20, 40, or 60 Gy. For each radiation condition, samples were evaluated either without treatment or after application of 600 µM myricetin for 120 seconds. Mechanical behavior was assessed by three-point bending tests, providing flexural strength, strain, and elastic modulus values. Enzymatic degradation was evaluated through a fluorometric assay measuring total MMP activity in dentin. Adhesive performance was analyzed using microtensile bond strength testing of resin–dentin interfaces, performed both at 24 hours and after thermocycling, along with failure mode classification. The data show a clear dose-dependent effect of radiation, characterized by significant reductions in flexural strength, elastic modulus, and microtensile bond strength, accompanied by increased dentin deformation and marked activation of MMPs, particularly at 60 Gy. Myricetin-treated specimens demonstrated partial or complete recovery of biomechanical parameters, significant suppression of MMP activity, and preservation of adhesive performance comparable to non-irradiated controls, even after aging. Failure mode distributions shifted toward cohesive failures within the resin in myricetin-treated groups, indicating improved interfacial integrity. All raw data are provided in spreadsheet format, organized by experimental group and outcome variable, allowing independent statistical reanalysis or secondary use. This dataset can be used to explore radiation-induced alterations in dentin biomechanics, enzymatic activity, and adhesion, as well as to support the development and validation of biomodification strategies aimed at improving restorative outcomes in irradiated dental tissues.本数据集旨在验证下述假说：头颈癌放疗中使用的电离辐射会损害人类牙根牙本质的机械、酶学与黏附性能，而天然存在的黄酮类化合物杨梅素（myricetin, MYR）可通过抗氧化、抗基质金属蛋白酶（matrix metalloproteinase, MMP）以及胶原稳定的机制缓解这些有害效应。 本数据集包含基于体外实验模型获取的定量测量数据，实验采用人类第三磨牙。牙根牙本质标本被分为未照射对照组，以及接受单次累积剂量20、40或60 Gy照射的照射组。针对每种辐射条件，样本分别接受无处理，或600 µM杨梅素处理120秒。通过三点弯曲试验评估机械性能，获取弯曲强度、应变与弹性模量数值。采用荧光分析法检测牙本质中总基质金属蛋白酶活性，以评估酶降解情况。针对树脂-牙本质界面的微拉伸粘结强度试验被用于分析黏附性能，试验分别在24小时及热循环后开展，并伴随失效模式分类。 数据显示辐射存在明确的剂量依赖性效应：弯曲强度、弹性模量与微拉伸粘结强度显著降低，同时伴随牙本质变形加剧与基质金属蛋白酶活性显著升高，尤其在60 Gy剂量组。经杨梅素处理的标本显示出生物力学参数的部分或完全恢复，基质金属蛋白酶活性得到显著抑制，且黏附性能得以保留，可媲美未照射对照组，即使经过老化处理亦是如此。杨梅素处理组的失效模式分布向树脂内聚失效偏移，这表明界面完整性得到改善。 所有原始数据均以电子表格格式提供，按实验组与结局变量进行组织，支持独立的统计学再分析或二次利用。本数据集可用于探索辐射诱导的牙本质生物力学、酶活性与黏附性能改变，也可用于支撑旨在改善受照射牙科组织修复效果的生物改性策略的开发与验证。