EASI-FISH example data (multi-round FISH, round 5)

Example dataset that can be used for testing the EASI-FISH (Expansion-Assisted Iterative Fluorescence in situ hybridization) data analysis pipeline (https://github.com/JaneliaSciComp/multifish). Raw image data (.czi) and metadata (.mvl) are provided here. Image data was collected using a Zeiss Lightsheet Z.1 microscope. A 20× water-immersion objective (RI=1.33) was used for imaging with 1× zoom. Single-side illumination was used to reduce light exposure and imaging time. Overlap between tiles was set to 8%. Voxel size (µm): 0.23 x 0.23 x 0.42 (x, y, z). It can be used with EASI-FISH example data (multi-round FISH, round 3 https://doi.org/10.25378/janelia.13622819) for registration testing. LHA3_R5_medium (32GB): Two image tiles are included in a single CZI file, with 4 channels (3 FISH channels and 1 DAPI channel). Channel1: 488 (Gad1), channel 2: 669 (Col25a1), channel 3: 405 (DAPI), channel 4: 546 (Synpr) and image dimension per tile: 1920 x 1920 x 575 (x, y, z). LHA3_R5_small (11GB): Two image tiles are included in a single CZI file, with 2 channels (1 FISH channel and 1 DAPI channel). Channel1: 488 (Gad1), channel 2: 405 (DAPI) and image dimension per tile: 1920 x 1920 x 414 (x, y, z).本数据集可用于测试EASI-FISH（Expansion-Assisted Iterative Fluorescence in situ hybridization，扩展辅助迭代荧光原位杂交）数据分析流程（https://github.com/JaneliaSciComp/multifish）。本数据集提供原始图像数据（格式为.czi）与元数据（格式为.mvl）。图像数据采用蔡司（Zeiss）Lightsheet Z.1光片显微镜采集，使用20×水浸物镜（折射率为1.33），放大倍率为1×。采用单侧照明以降低光暴露并缩短成像时长。图像拼接块间的重叠率设为8%。体素尺寸（单位：μm）为0.23×0.23×0.42（x、y、z轴）。 该数据集可搭配EASI-FISH示例数据集（多轮荧光原位杂交，第3轮，https://doi.org/10.25378/janelia.13622819）用于配准测试。 LHA3_R5_medium（32GB）：单个CZI文件中包含2个图像拼接块，共4个通道（3个荧光原位杂交通道与1个DAPI通道）。通道1：488 nm激发（标记Gad1），通道2：669 nm激发（标记Col25a1），通道3：405 nm激发（标记DAPI），通道4：546 nm激发（标记Synpr）；每个拼接块的图像维度为1920×1920×575（x、y、z轴）。 LHA3_R5_small（11GB）：单个CZI文件中包含2个图像拼接块，共2个通道（1个荧光原位杂交通道与1个DAPI通道）。通道1：488 nm激发（标记Gad1），通道2：405 nm激发（标记DAPI）；每个拼接块的图像维度为1920×1920×414（x、y、z轴）。