Effect of masitinib on the activity of protein kinases.

Recombinant tyrosine kinase assays were performed using an ELISA-based assay with poly(Glu,Tyr, 4∶1) as the substrate. All protein kinases were human versions except where noted. Cell-based assays were performed using Ba/F3 cells expressing the various enzymes, and cell proliferation was assessed using WST-1. All concentrations were tested in duplicate (recombinant assays) or triplicate (cell-based assays). Results are the means±standard deviations from at least three independent experiments except where noted. *Results are from a single experiment performed as part of a kinase screen by Proqinase (Germany).