Treatment for biofilm-related Staphylococcus aureus prosthetic joint infection

In-vitro antibacterial and antibiofilm activity of savirin or ticagrelor, alone and in combination with cefazolin, vancomycin, and rifampicin, against Staphylococcus aureus was tested. Effect of savirin or ticagrelor on the expression of key biofilm-related genes in S. aureus was also studied. Finally effect of savirin or ticagrelor, alone and in combination with cefazolin, to treat biofilm-related S. aureus prosthetic joint infection was studied in a mouse model. These experiments were performed to collect data to study the in-vitro antibacterial and antibiofilm activity of savirin or ticagrelor, alone and in combination with antibiotics, to study the effect of savirin or ticagrelor in the expression of key biofilm-related genes in S. aureus, and to study the effectiveness of savirin or ticagrelor, alone and in combination with cefazolin, to treat biofilm-related S. aureus prosthetic joint infection in an animal model. The antibacterial and antibiofilm activity of human synovial fluid against Staphylococcus aureus was also studied. The data generated were stored and analysed using graphpad. One way ANOVA followed by Tukey multiple comparison test was used.
Software/equipment used to create/collect the data: GraphPad version 8.2.0
Software/equipment used to manipulate/analyse the data: GraphPad version 8.2.0

本研究检测了沙维林（savirin）或替格瑞洛（ticagrelor）单用，以及与头孢唑林（cefazolin）、万古霉素（vancomycin）、利福平（rifampicin）联合使用时，对金黄色葡萄球菌（Staphylococcus aureus）的体外抗菌及抗生物膜活性。此外，本研究还探讨了沙维林或替格瑞洛对金黄色葡萄球菌（S. aureus）关键生物膜相关基因表达的影响。最后，本研究在小鼠模型中探究了沙维林或替格瑞洛单用，以及与头孢唑林联合使用时，治疗生物膜相关性金黄色葡萄球菌假体关节感染的效果。
本研究开展上述实验，旨在获取三类数据：一是沙维林或替格瑞洛单用及与抗生素联合使用时的体外抗菌与抗生物膜活性；二是沙维林或替格瑞洛对金黄色葡萄球菌关键生物膜相关基因表达的影响；三是沙维林或替格瑞洛单用及与头孢唑林联合使用时，在动物模型中治疗生物膜相关性金黄色葡萄球菌假体关节感染的有效性。本研究同时探究了人类滑液对金黄色葡萄球菌的抗菌及抗生物膜活性。
本研究采用GraphPad 8.2.0版本软件对所生成的数据进行存储与分析，统计分析方法选用单因素方差分析（one-way ANOVA）后行Tukey多重比较检验。
数据生成/采集所用软件/设备：GraphPad 8.2.0版本
数据处理/分析所用软件/设备：GraphPad 8.2.0版本