Experimental and analytical ToF-SIMS procedures of measurement data for membranous structure - research data

For ToF-SIMS measurements, silicon wafers (cat. No. 647780, Sigma Aldrich, St. Louis, MO, USA) with dimensions of 1 x 1 cm^2 were prepared. The selection of the silicon substrate was dictated by previous studies examining the best parameters for ToF-SIMS research on biomaterials. Before cell deposition, each substrate was sonicated in toluene (cat. No. 244511, Sigma Aldrich) and ethanol (absolute, 99%, cat. No. 396480111, POCH) for 10 min using an ultrasonic bath, rinsed with deionized water, and then dried with N2. The experimental material included 4 samples placed on the silicon surface with a volume of 30 µl. The concentration of deposited cells was approximately 5.3 x 105 cells/ml.