Capturing human trophoblast development with naive pluripotent stem cells in vitro [scRNA-Seq]

We report global transcriptional alteration and transcriptional trajectories from naive human pluripotent stem cells-derived trphectoderm to cytotrophoblast. By graph-based cell identities of total samples on the Seurat platform, we identified 9 main clusters, and further revealed the pseudotime differentiation during trophectoderm-cytotrophoblast transition. Overall design: Analyzing transcriptional profiles during naive human pluripotent stem cells-derived trophectoderm-cytotrophoblast transition using scRNA-seq. We generated chromium single cell 3' v3.1 libraries from randomly captured trophoblast-like cells and sequenced them using Illumina HiSeq 4000. Finally, we profiled 14,164 cells (nTE_D2: 2,378 cells; nTE_D3: 5,148 cells; nCT_D5:3,529 cells; nCT_D10: 3,109 cells) with a range of 56,312 – 130,971 mean reads per cell for each sample, whereby approximately 5,500–8,000 median genes per cell for each sample were detected.