Significant open-reading frame changes due to serial passage.

aThe “In-frame ORF tract length” was defined as the most frequently observed homopolymeric tract length that generated the longest potential open-reading frame. All tract lengths that introduced a premature stop codon were considered frameshifted.bA tract with length 10 increases in frequency during passage, but no ORF change could be detected since the tract is upstream of the predicted coding region.cThe tract lengths observed for Cj0046, Cj1295, and Cj1429c each contained a single read that differed from the “In-frame ORF tract length” by 3 guanines (or cytosines), and these were considered in-frame since they do not introduce a premature stop codon. A positive fold change indicates an increase of the in-frame ORF in mouse-adapted C. jejuni, and a negative fold change indicates a decrease.